Nociceptin-induced apoptosis in leukemia cell K562.docVIP

 PAGE \* MERGEFORMAT 4 Nociceptin-induced apoptosis in leukemia cell K562 Authors: GUO Hong-Zhao Li Baohong Juan Xuan Chen [Abstract] This study investigated the orphanin FQ on the K562 leukemia cell proliferation and induction of apoptosis, using MTT (tetrazolium) assay with different concentrations of orphanin FQ on the K562 cell growth, Application Wright stain , electron microscope observation of drug effect change in the structure after the K562 cells, flow cytometry of orphanin FQ on the K562 cells, apoptosis, DNA agarose gel electrophoresis the role of orphanin FQ K562 cells after the extent of DNA damage. The results showed that: 10-6-10-13 mol / L OFQ significantly inhibited the growth of K562 cells, and the existence of time-dependent, but not obvious dose-dependent manner; 10-6-10-7,10-9,10 -- 12 mol / L OFQ for 72 hours showed significant cytotoxicity. Compared with the control group ,10-9 mol / L OFQ for 72 hours after K562 cells were typical apoptotic features; detected by flow cytometry: 0,10-7,10-8,10-9 mol / L orphanin for 72 hours after apoptotic peak, the apoptosis rate were 0%, 22.8%, 23.8%, 26.5%; DNA agarose gel electrophoresis showed typical DNA ladder. Conclusion: OFQ can inhibit the proliferation of K562 cells and induce their apoptosis. [Keywords:] orphanin FQ; leukemia; apoptosis; K562 cells Apoptosis of K562 Cells Induced by Nociceptin / Orphanin FQ AbstractThe study was to investigate the prolilferation and apoptosis effect of nociceptin / orphanin FQ (OFQ) on 562 cells in vitro. Inhibition of K562 cells prolilferation was measured by MTT assay. Morphological assessment of apoptosis was performed with Wright staining and transmission electron microscope. The apoptosis peak was measured by flow cytometry. DNA fragmentation was visualized by agarose gel electrophoresis. The results showed that OFQ time-dependently and no-dose-dependently inhibited the proliferation of K562 cells at concentrations of 10-6-10-13 mol / L . Discret