Non-CO2-dependent cell culture system for PC3 prostate cancer cell proliferation.docVIP

Non-CO2-dependent cell culture system for PC3 prostate cancer cell proliferation [Abstract] Objective To investigate the non-CO2-dependent prostate cancer cell culture system for the proliferation of PC 3. Methods of prostate cancer PC 3 cells to non-CO2-dependent cell culture system and the CO2-dependent cell culture system for culture, are in the 37 incubator (atmosphere), and 37 , 50mL / L CO2 incubator conditions, the detection of pH changes in two systems; using cell growth curve and colony formation assay cells variance; and detected using RT PCR analysis under the two systems of prostate cancer cells survivin, caspase 3 expression. The results of the two culture systems under the conditions of cell culture medium pH the same trend (Pgt; 0.05); two culture systems is not the proliferation of PC 3 cell growth see the difference (Pgt; 0.05); RT PCR assay showed that the expression of two culture systems survivin, caspase 3 was no difference (Pgt; 0.05). Conclusion of non-CO2-dependent prostate cancer cell culture system on cell proliferation, gene expression, etc. and CO2-dependent prostate cancer cell culture system was no significant difference. [Keywords:] PC 3 prostate cancer cells; non-CO2 dependence; survivin; caspase 3 Methods Prostate carcinoma cell line PC 3 was cultured using CO2 independent culture cell system and CO2 dependent culture cell system at 37 in 50mL / L carbon dioxide incubator and at 37 in air incubator. The pH of culture medium with the two culture cell systems was detected. Differences in cell viability were assayed with CCK 8 test and colony forming assay. The expressions of survivin and caspase 3 mRNA of cell culturing with the two systems were detected by RT PCR assay. Results Under the two culture cell systems, the pH trend was the same (Pgt; 0.05), PC 3 cell proliferation and the expressions of survivin and caspase 3 mRNA of the cell had no obvious differences (Pgt; 0.05). Conclusion CO2 independent culture cell system