Normal with different syndromes H22 tumor-bearing mice with high expression of genes adrenal.doc

 PAGE \* MERGEFORMAT 24 Normal with different syndromes H22 tumor-bearing mice with high expression of genes adrenal Author: Pan Zhiqiang Zhao-Qin Fang Lu Mei-li Liang Chao Wu Zhonghua Liu Shao-Yuan Liao Mingjuan Hou Li Zhang Huizhuo Bi-Feng Gao [Abstract] Objective: To reveal the normal with different syndromes of adrenal tumor-bearing mice of Kunming species highly expressed genes. Methods: Kunming kinds of normal mice inoculated with the armpit H22 ascites tumor cells in mice, take the 8th day after tumor inoculation (early) cult poison Yongsheng syndrome and deficiency of Qi, the first 21 days (mid-term) yang deficiency, the first 29 days (the late) Gas Yin and Yang deficiency of tumor-bearing mice and normal control mice, adrenal gland, extracted RNA, purification and Gene Chip Mouse Exon 1.0 ST Array detection. Results: The screened 402 highly expressed genes, of which one or more groups-chip readings greater than the 5000 calculated values are 88. These genes are mainly involved in the regulation of metabolism and gene expression, some involved in the chemical synthesis, chemical processing modification, transport and signal transduction, as well as some involved in immunity, cell cycle and apoptosis, development; while many unknown gene functions. Conclusion: These in normal adrenal glands in mice with disease continuous and stable expression of genes may be in maintaining the normal physiological function of adrenal gland has an important biological significance; view of deficiency of Qi, tumor-bearing mice the expression of these genes are mostly lower than normal mice and the earlier cult poison Yongsheng certificate mice, reflecting the nature of qi deficiency syndrome and features; again, four different syndromes adrenal tumor-bearing mice of these differences in gene expression were focused on the trend confirms medicine ‘with the disease iso-certificate ‘theory and the nature of demand Differential Treatment. [Keywords:] adrenal gland; mouse;