Of aldosterone on glomerular mesangial cell synthesis and secretion of fibronectin and TGF.doc

 PAGE \* MERGEFORMAT 9 Of aldosterone on glomerular mesangial cell synthesis and secretion of fibronectin and TGF [Keywords:] aldosterone;, glomerular mesangial cells;, fibronectin;, transforming growth factor-β 1 Effects of aldosterone on synthesis of fibronectin and expression of transforming growth factorβ 1 mRNA in cultured rat mesangial cells [Abstract] AIM: To evaluate the effect of aldosterone (ALD) and spironolactone (SPI, one of the aldosterone receptor antagonists) on synthesis and secretion of fibronectin (FN) and expression of transforming growth factorβ 1 (TGFβ 1) mRNA in cultured rat mesangial cells . METHODS: (1) Mesangial cells were treated with medium containing different concentrations of ALD (10-11, 10-9, 10-7 mol / L) and / or 10-7 mol / L SPI for 48 h, while control cells were treated with vehicle only. The levels of FN in the supernatants were measured by ELISA method. The expressions of FN mRNA and TGFβ 1 mRNA were detected by semiquantitative RTPCR. (2) Mesangial cells were treated with 10-9 mol / L ALD for 24 , 48, 72 h. The levels of FN in the supernatants were measured by ELISA method. The expressions of FN mRNA and TGFβ 1 mRNA were detected by semiquantitative RTPCR. RESULTS: ELISA method showed that the level of FN in the supernatant of cultured rat mesangial cells stimulated with ALD increased significantly in a dose and timedependent manner. Also the expressions of FN mRNA and TGFβ 1 mRNA were increased significantly by ALD in a dose and timedependent manner by semiquantitative RTPCR. SPI inhibited the stimulating effect of ALD on synthesis of FN and expressions of FN mRNA and TGFβ 1 mRNA in cultured rat mesangial cells. CONCLUSION: ALD upregulates the protein synthesis and mRNA expression of FN, and upregulates the mRNA expression of TGFβ 1 in cultured rat mesangial cells. SPI inhibits the effect of ALD, and thus implication in the treatment of Glomerulosclerosis. [Keywords] aldosterone; mesangial cells; fibronectin; TGFβ 1