Of milrinone on pigs after cardiopulmonary bypass myocardial β -adrenergic receptor function in.doc

 PAGE \* MERGEFORMAT 6 Of milrinone on pigs after cardiopulmonary bypass myocardial β -adrenergic receptor function in [Abstract] Observation of milrinone on long-bypass (extracorporeal circulation, ECC) myocardial β -adrenergic receptor function. Methods 12 healthy domestic pigs were randomly divided into two groups, milrinone group and the control group 6. Established under general anesthesia ECC, the maintenance of cardiac arrest and 180 min. Open cardiac resuscitation aorta after the milrinone group were given milrinone loading 37.5μ g/kg, followed by 0.375μ g / (kg * min) maintained the same rate of the control group Zeyi intravenous saline. Disable the experiment catecholamine vasoactive drugs, fluid replacement and circulatory support through the maintenance of mean arterial pressure (MAP) is relatively stable. ECC, respectively, prior to cardiac resuscitation and medical treatment of atrial tissue specimens from 1 h to measure myocardial changes in cAMP and β -receptor activity. Results ECC myocardial cAMP content, β receptor activity significantly decreased than those in ECC, 1 h after drug treatment, the control group remained at a low level, milrinone significantly increased myocardial cAMP levels, β receptor activity is almost restored. ECC a long time after the conclusion of myocardial β -adrenergic receptor activity decreased, milrinone after ECC β -adrenergic receptor can promote functional recovery. [Keywords:] Milrinone pump adrenergic β -receptor Abstract: OBJECTIVE To investigate the effects of milrinone on myocardial β -adrenergic receptor (β -AR) function after extracorporeal circulation (ECC). METHODS 12 pigs were randomly divided into two groups, milrinone group and control group. ECC was established and aortic cross clamping was maintained for more than 180 minutes under general anesthesia. After aortic declamping, a bolus of milrinone 37.5μ g/kg was given within 10 minutes, followed by a continuous infusion of 0.375μ g/kg in milri