Old and young rats after intracerebral hemorrhage compared microglial reaction.docVIP

 PAGE \* MERGEFORMAT 12 Old and young rats after intracerebral hemorrhage compared microglial reaction [Abstract] Objective To investigate the age of cerebral hemorrhage microglia responses. Approach the young (3 months old) and old (16-month-old) male SD (Sprague-Dawley) rats (Experimental Animal Center of Henan Province ) intracerebral injection of autologous whole blood 100l observed age-related response of glial cells. young, old model, respectively, in the brain of rats after blood injection 6,12,24,72 h, 7,14 d time animals were sacrificed (n = 6) were OX42 immunohistochemistry. sham operation group (n = 6) as control. observe the expression of OX42 positive cells stained. The results compared with young rats, aged 3 d after cerebral hemorrhage with increased activation of microglia (OX42 staining). cortex, basal cells of microglia activation to determine their level of old rats in the cortex, basal ganglia more apparent activation of microglia. senile cerebral hemorrhage After 3 d OX42-positive cells was significantly higher than that of young rats, the situation to the monitoring of sustainable 14 d (P lt;0.01). Conclusion cerebral hemorrhage in the elderly is more serious brain injury in rats may be related to excessive microglia activation, age of cerebral hemorrhage is an important factor in brain injury. [Keywords:] cerebral hemorrhage; OX42; microglia; brain injury; rat Microglia may be ischemic, and hemorrhagic stroke damage and activation (1), into a phagocytic cell, clear cell debris from the site of injury. However, microglia also secrete many toxic substances like free radicals , may be involved in brain injury formation. research has shown that old age is affecting animal and human ischemic brain injury in an important factor in (2,3). but would not affect the aging brain hemorrhage (ICH) brain injury, currently no systematic research on aspects of animal models . to clarify the mechanism of aging brain injury after ICH ICH injury he