Olfactory ensheathing cells transplantation on rat brain AD COX activity and mitochondrial ultrastructure.docVIP

 PAGE \* MERGEFORMAT 17 Olfactory ensheathing cells transplantation on rat brain AD COX activity and mitochondrial ultrastructure Of: Yao Bochun Suntian Min Wang Jinyong Li Yong Wang Jun Li Wenchun with [Abstract] Objective To investigate olfactory ensheathing cells (OECs) transplantation for Alzheimer’s disease (AD) rat brain COX activity and mitochondrial ultrastructure. Methods SD rats with bilateral hippocampal injection of A1 ~ 40, to establish AD rat model. experimental animals were divided into four groups: normal control group, AD model group, OECs transplantation, artificial cerebrospinal fluid (MCSF) injection group, 12 in each group. OECs in primary culture in vitro and transplanted to the rat double-AD hippocampus. the use of behavioral test, histochemistry, in situ hybridization, and electron microscopy and image analysis techniques and tools to observe, compare learning and memory ability of rats, hippocampal CA1 area of mitochondrial cytochrome oxidase (COX activity and cytochrome Enzyme type subunits (CO ) mRNA expression and ultrastructure of neurons in CA1 and other indicators. Results compared with the control group, AD model rats, spatial learning and memory, hippocampal CA1 area of mitochondrial COX activity and CO mRNA expression was significantly lower (P lt;0.05), reduce the number of mitochondria, the structure is unclear, swelling, vacuolar degeneration, cristae rupture, OECs transplantation significantly increased the index (P lt;0.05), and mitochondrial ultrastructure of a clear protective effect . Conclusion AD OECs transplantation by improving the learning and memory in rats, increased hippocampal COX activity and CO mRNA expression and the protection of mitochondria effect on rat AD has obvious therapeutic effect. [Keywords:] Alzheimer’s disease, cytochrome oxidase, mitochondria, olfactory ensheathing cells transplantation [Abstract] Objective To observe the effect of olfactory ensheathing cells (OECs) transplantati