On the different processing methods on the fumarate content of active ingredients_0.docVIP

 PAGE \* MERGEFORMAT 14 On the different processing methods on the fumarate content of active ingredients Of: Cao Liu, Dou Zhiying, Wang Ping, Tian Yongliang [Abstract] Objective To compare the different processing methods on 3 kinds of fumarate content of the active ingredient. Methods of fresh Corydalis, boiled herbs Corydalis Corydalis, and are commercially available by Cuzhu, vinegar and wine Sunburn Sunburn after the ultrasound were used to water and methanol, heat reflux extraction method, using RP-HPLC determination of three kinds of effective components. The column was Agilent TC-C18 (250 mm × 4.6 mm, 5 μm), mobile phase: acetonitrile -0.1% phosphoric acid solution (triethylamine adjusted pH 5.3) to 28:72, flow rate: 1 mL / min, column temperature: 35 ℃, detection wavelength: 280 nm. After processing the results of fumarate by the water extracts were improved in the alkaloid content, alcohol content of alkaloid extract of an upward trend, Corydalis Sunburn fresh products directly through the vinegar, Cuzhu, wine Sunburn treatment, alkaline dehydrogenase Corydalis high content of fumarate in the origin of boiled tetrahydropalmatine higher levels. Conclusions In this study, the simultaneous detection of 3 kinds of active ingredients fumarate detection methods, this method is simple, reliable and repeatable. according to clinical need, Corydalis processing process can be boiled and then cooked up fresh goods or place of processing and integration of the two processing operation. [Keywords:] fumarate, protopine, dehydrogenase Corydalis base; Tetrahydropalmatine, high performance liquid chromatography, cooked Abstract: Objective To explore the effect of different processes on the contents of active ingredients in Rhizoma Corydalis. Methods Processes of vinegar-boiling, vinegar-frying and alcohol-frying were used to fresh corydalis, boiled corydalis and purchased corydalis. Then the method of extraction by ultrasound with water and by reflux with