Paeonia Tom Determination of berberine hydrochloride.docVIP

 PAGE \* MERGEFORMAT 10 Paeonia Tom Determination of berberine hydrochloride Of: Chen Lin, CYTOCHEMISTRY, Wang Ruihai, cattle Yanmei [Abstract] Objective To establish a peony soup in the determination of berberine hydrochloride. Methods using high performance liquid chromatography, column: Agilent XDB C18 (4.6 mm × 250 mm, 5 μm), mobile phase: acetonitrile -0.05 mol / L potassium dihydrogen phosphate - triethylamine (30:70:0.14), flow rate: 1.0 mL / min, column temperature: 25 ℃, detection wavelength: 345 nm. Results berberine range of 0.07 ~ 1.40 μg showed a good The linear relationship between the average recovery was 98.63%, RSD = 1.21% (n = 6), the average content of berberine was 1.55%. Conclusion The method is accurate, sensitive and reproducible for the quality control of Paeonia soup provided. [Keywords:] peony soup, hydrochloride, HPLC, determination Abstract: Objective To determine the content of berberine hydrochloride in Shaoyao decoction. Methods HPLC was performed on an Agilent XDB C18 column by using acetonitrile-0.05 mol / L potassium dihydrogen phosphate-triethylamine (30:70:0.14) as mobile phase at a flow rate of 1.0 mL / min, the column temperature was 25 ℃ and the UV detection wavelength was 345 nm. Results Berberine hydrochloride showed a good linearity redationship in the range of 0.07 ~ 1.40 μg. The average recovery was 98.63% (n = 6, RSD = 1.21%). The average content was 1.55%. Conclusion The method is simple and accurate, with good repeatability, and can be used for the quality control of Shaoyao decoction. Keywords:: Shaoyao decoction, berberine hydrochloride, HPLC, content determination Peony soup from lt;lt;Su asked pathogenesis gas should be a life-saving setgt;gt; [1], by the peony, angelica, berberine, baicalin, betel nut, wood, licorice, rhubarb, cinnamon, composed of 9 herbs, with a heat dampness, to reconcile the efficacy of blood, heat treating dysentery. peony soup for the common prescription treatment of