OXA50-type carbapenemase genes in Pseudomonas aeruginosa in the preliminary study of the distribution of.docVIP

 PAGE \* MERGEFORMAT 14 OXA50-type carbapenemase genes in Pseudomonas aeruginosa in the preliminary study of the distribution of Author: Liu Wei had medu Bao Xi Cheng Tao Chuan Wen-Xiang Jia Min Kangmei [Abstract] Objective To study the Pseudomonas aeruginosa (Pseudomonas aeruginosa, PA) to the carbapenem antibiotic resistance of the molecular mechanism. Methods of non-duplication of 34 clinical strains of Pseudomonas aeruginosa, PCR amplification blaOXA  50 gene sequencing, in GenBank than right, extracting plasmid DNA, the PCR gene amplification. The results of PCR detected 34 of Pseudomonas aeruginosa has 15 including OXA  50-type genes, including 30 imipenem-resistant Pseudomonas aeruginosa has 12 positive, there are three of four susceptible strains strains positive; randomly selected a send-positive bacteria sequenced PCR products, sequencing results in GenBank on the blast than the right, showed that two mutations points; blaOXA  50 PCR-positive 15 Pseudomonas aeruginosa, plasmid DNA extraction amplification of the gene, the result is negative. Conclusion This experiment examined 34 clinical strains of Pseudomonas aeruginosa, OXA  50-based gene detection rate of 44.10%, of which a point mutation found in two places; detected blaOXA  50 coding genes on chromosome on. [Keywords:] Pseudomonas aeruginosa; OXA  50-type gene; carbapenem antibiotics ABSTRACT Objective To study the distribution of OXA  50 gene in Pseudomonas aeruginosa. Methods A total of 34 isolated clinical strains of Pseudomonas aeruginosa were subjected to PCR analysis with primers specific for blaOXA  50, which was used for comparing the sequence results with corresponding sequences in GeneBank, then plasmid DNA of positive strains for PCR was extracted. Results 15 isolated strains showed positive results for blaOXA  50 detection, 12 strains were identified in carbapenems  resistant Pseudomonas aeruginosas, and 3 strains were detected in carbapenems  sensitive Pseu