Oxacillin against Staphylococcus aureus ATCC25923 penicillin-binding protein affinity studies.docVIP

 PAGE \* MERGEFORMAT 11 Oxacillin against Staphylococcus aureus ATCC25923 penicillin-binding protein affinity studies Paper Keywords oxacillin affinity penicillin-binding proteins Abstract using sodium dodecyl sulfate - polyacrylamide gel electrophoresis, fluorescence autoradiography and competition assay oxacillin against Staphylococcus aureus ATCC25923 penicillin-binding protein affinity. The results showed that there are five Staphylococcus aureus penicillin-binding proteins, namely, PBP1, PBP2, PBP3, PBP4, PBP5, molecular weight range of 41 ~ 87kD. Oxacillin mainly Staphylococcus aureus ATCC25923 strong affinity of PBP1 and PBP2, especially with a strong affinity PBP2. It showed Staphylococcus aureus in PBP1 and PBP2 is the main role of the target site oxacillin. Penicillin-binding protein (penicillin-bindingproteins, PBPs) are present in bacterial cells lining the group to work with penicillin and other β -lactam antibiotics of bacterial protein chelate, that is, the role of the target sites of antibiotics, antibiotics with these target site points combined, disrupted bacterial cell wall peptidoglycan synthesis of bacterial cell lysis caused by the death. Oxacillin (oxacillin) against Staphylococcus aureus has a strong antibacterial effect, its mechanism of action studies, domestic not been reported. In this paper, microbiological and biochemical research methods, from the standard strain ATCC25923 Staphylococcus aureus bacteria extracted endometrial proteins, using sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS), fluorescence autoradiography and competition analysis study of Staphylococcus aureus oxacillin and the standard strain ATCC25923 cells lining the affinity penicillin-binding proteins to explore the role of oxacillin bactericidal molecular basis. 1 Materials and methods 1.1 Drugs and reagents Oxacillin provisional standard for the country, 910u/mg; melting aureus hormone (lysostaphin) for Sigma’s products; - penicillin G po