Peripheral blood of patients with acute myeloid leukemia in the expression of RIZ1 promoter methylation and associated research.docVIP

 PAGE \* MERGEFORMAT 16 Peripheral blood of patients with acute myeloid leukemia in the expression of RIZ1 promoter methylation and associated research Of: Yao Chen, Fang Juanjuan, high Lili, Jia-Hua Ding, bundles defense, Yu Weiping [Abstract] Objective: To study the peripheral blood of patients with acute myeloid leukemia tumor suppressor gene status and expression of the RIZ1 gene promoter methylation status and the relationship between the two. Methods: 37 patients with primary acute myeloid leukemia patients and 15 healthy blood samples for the study. RT PCR detection of RIZ1 mRNA level, methylation specific PCR (MSP) detect RIZ1 gene promoter methylation status. Results: The patients with acute myeloid leukemia and normal samples Comparison of human specimens, RIZ1 gene expression decreased, the difference was statistically significant (P lt;0.05). acute myeloid leukemia samples RIZ1 gene promoter methylation was 29.7% (11/37). there is methylation peripheral blood of patients with acute myeloid leukemia samples, RIZ1 gene is missing or reduced. Conclusion: RIZ1 gene expression decreased with the occurrence of acute myeloid leukemia is related to some of its mechanisms is gene promoter methylation. [Keywords:] Acute myeloid leukemia; RIZ1 gene; DNA methylation [Abstract] Objective: To investigate the expression of RIZ1 gene in acute myeloid leukemia and to analyze the relationship between this alteration and the promoter hyper methylation of RIZ1 gene. Methods: The expression of RIZ1 mRNA in peripheral blood from 37 patients of primary acute myeloid leukemia samples and in peripheral blood from 15 normal person s samples was detected by reverse transcripition polymerase chain reaction (RT PCR). Methylation specific PCR (MSP) was used to assay the methylation of RIZ1 gene promotor region. Results: Compared with normal person , the RIZ1 gene expression level decreased significantly in acute myeloid leukemia (P lt;0.05). The Methylation rate was