Peripheral blood CK20 mRNA expression and colorectal cancer diagnosis staging and prognosis of.doc

 PAGE \* MERGEFORMAT 5 Peripheral blood CK20 mRNA expression and colorectal cancer diagnosis staging and prognosis of [Keywords:] colorectal cancer cytokeratin reverse transcriptase-polymerase chain reaction  0 Introduction We used RT  PCR technique to detect 126 cases of colorectal cancer in patients with peripheral blood mononuclear cells CK  20 mRNA expression to study the CK  20 mRNA expression in colorectal cancer clinical significance. 1 Data and methods 1.1 The object of study 1.1.1 colorectal cancer confirmed by pathology after surgery in 126 cases of colorectal cancer patients, male 75 cases, female 51 cases, aged 38 to 73 years, mean 57.4 years old; according to Duke’s staging: A period of 23 cases, B phase 34 cases , C period of 44 cases, D period of 25 cases. Peripheral blood collected before surgery. 1.1.2 normal control group of healthy volunteers, 25 cases, 17 were males and 8 females, aged 29 to 58 years, an average of 46.5 years. 1.1.3 cell line group ① HT29 cell line (colon cancer cell line); ② H740 cell line (HCC cell line); ③ 2HTB  133 (breast cancer cell line); ④ TPG5 (lung cancer cell line); ⑤ K562 cell line (red white blood cell disease cell line); ⑥ SGC7901 (gastric cancer cell line), conventional cell culture. 1.1.4 Other group of gastric cancer, lung cancer, liver cancer, breast cancer, esophageal cancer of 10 cases. 1.2 Methods 1.2.1 Sample collection and preservation of (1) Blood samples should be taken by patients with colorectal cancer and normal blood donor peripheral blood 10ml, into the 20u/ml heparin anti-coagulation treatment in vitro, the isolated mononuclear cells into  80 ℃ refrigerator prepare test. (2) a source of solid tumor specimens of colorectal cancer and other cancers, colorectal cancer and other cancers from patients with cancer after surgery. Samples sent to laboratory within 30min, fresh extract total RNA; can not guarantee that the samples prepared within 30min, in the spe