PI3K-Akt inhibitor cisplatin Experimental Study of Lung Cancer.doc

 PAGE \* MERGEFORMAT 15 PI3K/Akt inhibitor cisplatin Experimental Study of Lung Cancer Of: Charles Ng Miao Guang Song Lijun Wang Jing Chiang [Abstract] Objective To investigate the PI3K/Akt inhibitor LY294002 (2 (4-phenyl-morpholinyl 8 4 H 1 benzopyran 4 one) combined with chemotherapy drug cisplatin (DDP treatment effects and possible mechanisms of lung cancer to lung cancer clinical basis for new theories. Methods Cultured lung adenocarcinoma A549 cells by MTT method and flow cytometry combined DDP LY294002 on proliferation and apoptosis of A549 cells affected by nude mice assay on A549 DDP combined LY294002 cells into nude mice. results in a certain dose range, LY294002 and DDP could inhibit the growth of A549 cells, the inhibition was dose-effect relationship. LY294002 combined with DDP can enhance the role of inhibition of A549 cells. LY294002 and DDP can be effective in inducing apoptosis in A549 cells, together with the apoptosis rate was significantly increased. xenografts in nude mice experiments showed that, LY294002 and DDP could inhibit tumor growth, combined with inhibition rate after the increase. Conclusion LY294002 can enhance DDP on A549 cells, nude mice of anti-tumor effect, the effect may be enhanced on the A549 through the inhibition of cell proliferation and induced apoptosis to achieve. [Keywords:] lung cancer, apoptosis, PI3K, LY294002, cisplatin Cell survival signal transduction on the development of lung cancer plays a vital role in .PI3K/Akt signaling pathway cancer survival in a foreign country is considered the primary pathway. In recent years, the study found that of Akt, Akt through phosphorylation many of its downstream substrate for the resistance to chemotherapy and radiotherapy .PI3K/Akt inhibitor LY294002 (2 (4 up Lin Ji) 8-phenyl-4 H 4 1 Benzo adjacent furan ketone) can enhance some of the anti-tumor drug efficacy and reduce resistance to chemotherapy (1,2). LY294002 for lung cancer research while still in the ini