Pigmented choroidal neovascularization in rabbit connective tissue growth factor expression in.doc

 PAGE \* MERGEFORMAT 17 Pigmented choroidal neovascularization in rabbit connective tissue growth factor expression in Author: Tan Dongju, Luo-Sheng, Liu Xiangping, Tang Zhen 【Abstract】 Objective: pigmented rabbits by laser-induced choroidal neovascularization (choroidal neovascularization, CNV) model to explore the connective tissue growth factor (connective tissue growth factor, CTGF) in the role of CNV formation. Methods: Using high-strength semiconductor-pumped frequency-doubling Nd: YAG 532nm green laser photocoagulation of retinal posterior pole, induced CNV. 1,3,4 wk after in situ hybridization detection of CTGF mRNA expression in the CNV. Results: The day after photocoagulation 1wk, CNV membrane began to take shape, CTGF mRNA was mainly expressed in CNV and its vicinity; photocoagulation after 3,4 wk, CTGF mRNA expression was still evident and has enhanced the trend. Conclusion: The connective tissue growth factor may be involved in the occurrence and development of experimental CNV. 【Key Words】 choroidal neovascularization 0 Introduction Macular choroidal neovascularization (choroidal neovasculariz-ation, CNV) membrane formation is age-related macular degeneration (age-rela-ted macular degeneration, AMD), a high degree of myopia and other ocular fundus diseases cause vision loss the most important reason. Experimentally induced and surgical removal of neovascular membrane histological and immunohistochemical study confirmed that a variety of growth factors including vascular endothelial growth factor (VEGF), transforming growth factor β (TFG-β) and other catalytic role in the formation of CNV [1-5 ]. Connective tissue growth factor (connective tissue growth factor, CTGF) is a recently reported highly conserved immediate early genes CNN (CTGF, cyr, nov) family, one of its wound healing and fibrosis play a major role [6 -- 8]. Recent studies suggest that CTGF in promoting fibrosis, but also to promote angiogenesis in vi