Pill rat thymocyte apoptosis and Bcl-2 Bax expression.doc

 PAGE \* MERGEFORMAT 21 Pill rat thymocyte apoptosis and Bcl-2 Bax expression Of: the early product Gongzhang Bin Xu Jin Guoqin [Abstract] Objective To observe the intervention of corticosterone Pill induced thymocyte apoptosis and Bcl-2, Bax expression. Method 1 10-5mol / L corticosterone treatment neonatal rat thymus cells. Were divided into normal control group, model group, the left grouping. Annexin V-PI double staining method with FCM detection of thymocyte apoptosis; Real time PCR and Western blot Bcl-2, Bax expression. Results of thymus cells were treated with corticosterone and thymus cells was significantly higher (P lt;0.01); Bcl-2 mRNA expression was significantly decreased; Bax was significantly higher (P lt;0.01), Bcl-2/Bax ratio decreased (P lt;0.01). with model group Comparison of the left grouping thymocyte apoptosis rate (P lt;0.01); Bax mRNA expression decreased, Bcl-2/Bax ratio increased (P lt;0.05). Conclusion Zuoguiwan mouse serum through regulation bcl-2/bax The abnormal rate of change, inhibit excessive apoptosis of thymocytes. [Keywords:] thymus cells; Pill; apoptosis; Bcl-2; Bax [Abstract] ObjectiveTo investigate the accommodationand the molecular mechanism of Zuogui Pill (ZGP) on the apoptosis of rat thymocytes induced by corticosterone.MethodsThe ZGP contained rat serum were prepared. Thymocytes were divided into control, model, ZGP groups. The effect of apoptosis wasmeasured by Annexin V-FITC/PI double staining and FCM. The expressions of Bcl -2, Bax were measured by real-time PCR and Western blot.Results10-5 mol / L Corticosterone induced apoptosis of thymocytes, up-regulated the expression of Bax . The ratio of Bcl-2/Baxwas descended. Comparing with model group, ZGP group showed lower expression ofBax (P lt;0.05), the ratio of Bcl-2/Bax was elevated. The number of apoptosis cells were decreased (P lt;0.05 ). ConclusionsZGP contained rat serum can inhibit the apoptosis of thymocytes induced by corticosterone. The possi