Polygonum drink hypothalamus of female aging rats of neurotransmitter.docVIP

 PAGE \* MERGEFORMAT 14 Polygonum drink hypothalamus of female aging rats of neurotransmitter Study: LI Ya-li, Zhang Na, MATERIA, High Flow, Wangfang Na, Qi Xuan, Chen Xue [Abstract] Objective To investigate the hypothalamic neurotransmitter changes during aging and senescence Polygonum drink delaying mechanism of the hypothalamus. Methods D-galactose induced subacute intraperitoneal injection of aging model, after the model 60 d continuously fed Polygonum drink After decapitation remove the hypothalamus in rats. by ELISA assay of rats SP, -EP and GnRH contents, high performance liquid chromatography (HPLC with SHIMADZU-10A high performance liquid chromatography and L-ECD-6A electrochemical detector hypothalamic monoamine neurotransmitters NA, DA ,5-HT content. Results Compared with normal control group, model group hypothalamic GnRH, SP significantly increased, -EP and NA, DA ,5-HT content was decreased, the difference was statistically significant (P lt;0.05. Compared with model group, fleece-flower root drinking group of hypothalamic GnRH, SP significantly decreased, -EP and NA, DA ,5-HT was significantly increased a dose dependent manner. Conclusions of aging in rats the normal function of the hypothalamus is damaged, fleece-flower root drinking on the regulation of neurotransmitter disorder to delay the aging of the hypothalamus. [Keywords:] Polygonum drink, hypothalamus, neurotransmitter Abstract: ObjectiveTo investigate the changes of hypothalamus brain neurotransmitter of aging rats and mechanisms of Heshouwuyin (HSWY) on anti hypothalamus aging.Methods The sub-acutely aging model was continually induced by ip injection of D-galactose in rats. Oral HSWY was administered once daily for 60 days after aging induction.The rats were killed by being amputated by head and the hypothalamus was taken. Use the method of ELISA to detect SP, -EP and GnRH. The concentration of monamine transmitters including noradrenaline (NA), dopamine compared with the