Polygonum raw materials and processed products by TLC identification method of.doc

 PAGE \* MERGEFORMAT 9 Polygonum raw materials and processed products by TLC identification method of Of: Liuzhen Li, SONG Zhi ago, Mao Zhi Chao, Wang Chun, Lu Department I, Li Linfu [Abstract] Objective To study the use of thin layer chromatography (TLC) method of identifying differences between raw materials and processed products of Polygonum feasibility. Method of samples from different parts of the country’s three batches of fleece-flower root samples and 10 batches of the system Polygonum samples and authentic origin of raw materials through different processing Polygonum process, preparation of different processed products of processing time. TLC identification of conditions: silica gel G thin layer plate, 5 - hydroxymethylfurfural as reference to petroleum ether (60 ~ 90 ℃) - ethyl acetate (1:1) as developing solvent, sprayed with 15% @ - naphthol ethanol, heated at 90 ℃ to spot the color clear, daylight view. The results for the test products for chromatography, in the control Chromatography products corresponding position, the system was the same color spots Polygonum, Polygonum does not show the same color of the spots. Conclusion The TLC identification method to distinguish between raw materials and processed products of Polygonum. [Keywords:] Polygonum, TLC, 5 - hydroxymethylfurfural Abstract: Objective To study the method for distinguishing the crude and processed Radix of Polygonum multiflorum Thunb. By TLC. Methods Some of the samples were collected from different cities in China, some of them were prepared following the pharmacopoeia standard or local standard. TLC conditions :5-hydroxymethyl-furfural (5-HMF) was used as a standard matter with the developing solvent of petroleum ether (60 ~ 90 ℃)-ethyl acetate (1:1) on a silica G thin layer plate, sprayed with 15% @-naphthol in ethanol solvent, heated and detected in daylight. Results TLC chromatogram showed that crude and processed product of Polygonum multiflorum Thunb. were diffe