Polyketides and its combinatorial biosynthesis.doc

 PAGE \* MERGEFORMAT 37 Polyketides and its combinatorial biosynthesis Keywords: polyketides;,, polyketide synthase;,, combinatorial biosynthesis Abstract: The combination of polyketides biosynthesis is the current international cross-disciplinary study of chemistry and biology of one of the hot are also being developed into drug discovery and an important means of super-normal. In this paper, over the past decade polyketides, particularly the I-polyketides biosynthesis of the commonly used a combination of technical and methodological development of the review and prospects. Keywords: polyketides; polyketide synthase; combinatorial biosynthesis ABSTRACT Polyketide compounds and their biosynthesis by combinatorial approaches had received great attention as a crossdisciplinary subject in a new filed of chemical biology for its importance and great potential to create brand new drug in future. In this review, we hope to summarize methods and technologies which had been developed over past ten years or so for the imaginative discovery of novel polyketide compounds by combinatorial biosynthesis, which incorporate genetic, chemical and biochemical, bioinformatic and biotechnological approaches.KEY WORDS Polyketide; Polyketide synthase; Combinatorial biosynthesis 1 polyketides and polyketide synthase Polyketides are a large class by bacteria, fungi and plants will be low-carboxylic acid through successive condensation reaction of natural products, which includes many inhibit the bacteria (such as erythromycin, tetracycline), fungi (such as sallow sp Su, amphotericin), parasites (such as avermectin, Naima Kading), cancer (eg, doxorubicin, enediynes) and other active compounds, some anti-fungal polyketides also have immunosuppressant activity (such as the rapamycin, FK506), which is widely used in medicine, animal husbandry and agriculture. Now, this compound more and more people are paying attention, mainly due to the compound has: (1