Polynuclear leukocyte elastase on human corneal stromal cells in MMP1 MMP3 expression.doc

 PAGE \* MERGEFORMAT 9 Polynuclear leukocyte elastase on human corneal stromal cells in MMP1 MMP3 expression Of: Zhang Bingjie Haoji Long Lu Jia Wu Yazhen Abstract Objective To investigate the multi-core leukocyte elastase and interleukin (IL) 1 on human corneal stromal cells in MMP 1, 3 expression. Methods Cultured human corneal stromal cells in the culture medium with or without multinucleated leukocyte elastase or IL 1, cultured 5 d, determined by Western blot analysis of culture supernatant of MMP 1, 3 expression. The results in the absence of any stimulus Human corneal stromal cells did not express MMP 1, 3; added after induction of IL 1 precursor MMP 1, 3 expression, while adding multi-core leukocyte elastase did not induce MMP 1, 3 expression; polynuclear leukocyte elastase can activate the IL 1 production induced by the precursor MMP 1, 3 into the activated form of MMP 1, 3. Conclusion multicore leukocyte elastase and IL 1 synergistically induced corneal stromal cells in collagen degradation, induced corneal ulcers. Keywords: multi-core leukocyte elastase; interleukin 1; corneal stromal cells; MMP 1; MMP 3 Abstract Objective To understand the mechanism of corneal ulceration by investigating the role of neutrophil elastase (NE), interleukin (IL) 1 and corneal fibroblasts on expression of matrix metalloproteinases (MMP) 1 and 3 in cultured human corneal fibroblasts. Methods NE or IL 1 was added to culture medium of human corneal fibroblasts 5 d later. The activity of MMP 1 and 3 were examined by immunoblot analysis. Results Cultured human corneal fibroblasts didn’t release MMP 1 and 3 without any stimulation. IL 1 induced the expression of proMMP 1 and 3, NE transformed proMMP 1 and 3 into active forms induced by IL 1. Conclusions NE and IL 1 can co induce human corneal fibroblasts in collagen degradation . Keywords: Neutrophil elastase; Interleukin 1; Human corneal fibroblasts; MMP 1; MMP 3 Co