Poria Poria in different areas of comparative analysis of acid content.doc

 PAGE \* MERGEFORMAT 8 Poria Poria in different areas of comparative analysis of acid content Of: Zhaoying Bo, Xu Bin, Zan Junfeng, Su Wei, Wang Keqin, Liu Yan Wen [Abstract] Objective To Fuling Poria Determination of acid content, compared nine different areas in Poria cocos acid content, in order to evaluate the quality of different areas to provide reference Poria. Methods RP-HPLC (RP-HPLC ), Kromasil 100-5 C18 column (250 mm × 4.6 mm, 5 μm), column temperature 30 ℃, mobile phase of acetonitrile -0.4% acetic acid (80:20), flow rate 1.0 mL / min, detection wavelength 242 nm. Results Poria acid in the range 0.48 ~ 2.40 μg its chromatographic peak area was linear, r = 0.999 7, the average recovery rate of 99.96%, RSD = 1.85% .9 localities were Fuling Poria acid vary. Conclusion The method is simple, specific, stable, can be used for Poria Poria Radix acid content. [Keywords:] Poria, Poria acid, HPLC, determination Abstract: Objective To establish a method for determining the content of Pachymic acid in Poria cocos, and compare the content of Pachymic acid of Poria cocos from nine different areas, in order to provide a basis to evaluate the quality of Poria cocos from different areas. Methods RP-HPLC method was used on Kromasil 100-5 C18 column (4.6 mm × 200 mm, 5 μm), with acetonitrile-0.4% ethanoic acid (80:20) as mobile phase, flow rate of 1.0 mL / min, column temperature at 30 ℃, and detection wavelength at 242 nm. Results Pachymic acid showed a fine linear relationship with the peak areas between 0.48 ~ 2.40 μg (r = 0.999 7). The average recovery was 99.96% with RSD = 1.85%. There was a difference in the content of Pachymic acid in Poria cocos from nine areas. Conclusion The method is proved to be convinient, stable, and feasible for quality assessment of Pachymic acid which is the main effective content in Poria cocos. Keywords:: Poria cocos, Pachymic acid, RP-HPLC, content determination Division for the fungus Poria cocos fungus porous