Portulaca oleracea L. extract the liquid outside the antimicrobial effect of experimental study.doc

 PAGE \* MERGEFORMAT 10 Portulaca oleracea L. extract the liquid outside the antimicrobial effect of experimental study 【Abstract】 Objective To observe the Portulaca oleracea L. extract liquid antibacterial test results. Methods in clinical isolates of Shigella, Escherichia coli, Staphylococcus aureus, Enterococcus bacteria as subjects. Using geometric dilution Portulaca oleracea L. extract in vitro minimum inhibitory concentration (MIC). Results Purslane on Shigella, Escherichia coli stronger antibacterial effect, MIC ranges of 7.81 ~ 0.97 g * L-1, and 31.25 ~ 1.95 g * L-1, MIC50 and MIC90 were 3.90,7.81 g * L-1, and 7.81,31.25 g * L-1; pairs of Staphylococcus aureus and Enterococcus weak bacteriostatic effect, MIC range were 125.00 ~ 3.90 g * L-1 among, MIC50 and MIC90 were 31.25 and 62.50 g * L-1. Conclusion purslane right of Shigella and Escherichia coli has a strong antibacterial effect. Keywords: Portulaca antibacterial flavonoids pathogens Portulaca oleracea Portulacaceae Portulace oleracea L. is a plant, Alias: melon seeds food, long life food, climbing to Amaranthaceae, horse teeth dishes, is a whole herb, most of the distribution area are edible wild. “Compendium of Materia Medica” in early records in this regard, Li said: “there are many of purslane, field Yousheng ... ... more people to adopt sun as the vegetables cook seedlings.” Portulaca oleracea treatable diseases such as diarrhea card. This study antibacterial effect of Portulaca oleracea L. extracts designed to provide a theoretical basis for clinical application. 1 Materials and methods 1.1 Materials 1.1.1 for the test strain and medium experimental use G bacteria: Staphylococcus aureus and Enterococcus, G-bacteria: Shigella and Escherichia coli, all 20. Isolates were isolated from clinical cases of acquired in vivo. Ordinary nutrient agar medium are used. Ordinary nutrient agar medium, nutrient broth were purchased from Hangzhou Reagent Co., Ltd. m