Principles and Applications of RNA Interference.doc

 PAGE \* MERGEFORMAT 9 Principles and Applications of RNA Interference Abstract RNA interference is a post-transcriptional gene silencing in a way, is an ancient biosphere and the evolution of the phenomenon of highly conserved. RNAi mediated by siRNA-specific and efficient means of inhibiting gene expression by siRNA-mediated targeting to identify and target cutting homologous mRNA. RNAi has the characteristics of bio-catalytic reaction, the reaction need to participate in a variety of protein factors and ATP. RNAi in gene function and gene drug has broad prospects. Keywords: RNA interference siRNA dsRNA RNA-induced silencing complex Argonaute RNA polymerase III promoter 【Abstract】 RNA interference (RNAi) in diverse organisms reveals the same highly conserved mechanism with an ancient origin.RNAi is the mode of sequence-specific post-transcriptional gene silencing in animals and plants initiated by homologous double-stranded RNA (dsRNA). discovery of RNAi and the molecular mechanism will help us apply it to study the gene function and exploit the gene drug. 【Keywords:】 RNA interference (RNAi) small interfering RNA (siRNA) double-stranded RNA (dsRNA) RNA-induced silencing complex (RISC) Argonaute pol III 1 Background More than 20 years ago, in the Petunia hybrida (petunias) conducted a study there found that: Rich Jorgensen and colleagues will be able to produce a pigment gene placed in a strong promoter, the import petunia in an attempt to deepen the flowers The Color Purple, the results did not expect to see deep purple flowers, most flowers have become spotted or even white. Because the import of genes and their similarity to endogenous gene also have been inhibited, Jorgensen named this phenomenon synergistic suppression (cosuppression). In fungi have a similar phenomenon in 1996, in the Genus Neurospora (Neurospora) discovered this phenomenon. This phenomenon was named the genes in Table A of the inhibitory