Pulmonary microvascular endothelial cell phenotype changes of bleomycin-induced pulmonary fibrosis.docVIP

 PAGE \* MERGEFORMAT 6 Pulmonary microvascular endothelial cell phenotype changes of bleomycin-induced pulmonary fibrosis [Abstract] Objective: To observe the bleomycin-induced pulmonary fibrosis in rat microvascular endothelial cells (LMVECs) changes in ultrastructure and serum CTGF to explore the pulmonary microvascular endothelial cell phenotype and with pulmonary fibrosis in bleomycin injury among relationship. Methods: SD rats were 45, were randomly divided into 3 groups: bleomycin (BLM) group, Streptococcus pneumoniae group and the normal control group, n = 15, respectively, and pneumonia, tracheal instillation of bleomycin Streptococcus modeling, control group, normal saline drip. electron microscopic observation of endothelial cells in peripheral lung tissue ultrastructure, while serum CTGF levels. Results: Light microscopy and electron microscopy showed that pneumonia group and the different changes in BLM group. histology and patients with pneumonia Ultrastructure of pulmonary microvascular endothelial cells showed a typical acute inflammatory injury and repair, followed by the organizational structure of the lung returned to normal. BLM group showed an ongoing lung injury, pulmonary microvascular endothelial cell proliferation 7 d beginning and the emergence of morphological changes, and the various stages throughout the experiment and eventually marked deposition of collagen in the alveolar septum. CTGF in serum measured: pneumonia group 3 d began to increase, the first 7 d dropped to (5.04 ± 0.82) ng / L, the first 21 d restored to (12.06 ± 0.43) ng / L. BLM group increased throughout the first 7 d to (12.53 ± 2.36) ng / L, the first 21 d to the peak (19.45 ± 0.41) ng / L. Conclusion: The pulmonary microvascular endothelial cell function and secretion of CTGF, when the changes phase and may be abnormal amount of bleomycin-induced lung fibrosis in the development of an important part. [Keywords:] pulmonary fibrosis in bleomycin vascular endoth