Pulsatilla Trichomonas vaginalis in vitro differential expression of proteins.doc

 PAGE \* MERGEFORMAT 15 Pulsatilla Trichomonas vaginalis in vitro differential expression of proteins Of: Qiong, Huang Huicong, Liang Shao-Hui, Panchang Wang [Abstract] Objective: To find the specific proteins of Trichomonas vaginalis and to explore its protein differential expression of Pulsatilla. METHODS: The solid-phase pH gradient two-dimensional gel electrophoresis of total protein of Trichomonas vaginalis, the gel with silver staining color, PDQuest7.4.0 software analysis. Results: The average protein spots found in the control group (435 + -17 months, matching rate was 92.5%, Pulsatilla group of proteins found in the average (343 + -9 months, matching rate was 94.9%. characterization analysis, the normal group and the expression of Pulsatilla group consisted of 58 different points, which only expressed in the normal group, 45 points, only points in the expression of Pulsatilla group of 13, quantitative analysis, Pulsatilla group and the normal group, increased expression 2 times greater than the 43 points, 50% more points with 50. Conclusion: A normal group of Trichomonas vaginalis and Pulsatilla Two dimensional gel electrophoresis, identified 151 differentially expressed proteins, Pulsatilla kill Trichomonas vaginalis occurred in the course of the protein changes in quality or quantity. [Keywords:] Trichomonas vaginalis, Pulsatilla, proteome, two-dimensional gel electrophoresis Abstract: Objective: To search the characteristic proteins of Trichomonas vaginalis (T.vand explore the influence of Pulsatilla chinensis on discrepancy protein expression. Methods: The proteins of Tv were separated with immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE ). After silver staining, PDQuest 7.4.0 software was used to analyse 2-DE images. Results: About 435 + -17 and 343 + -9 protein spots were found in the two maps of normal group and Pulsatilla chinensis group, respectively: The match ratio was 92.5% and 94.9% respectively. Fifty-e