Rabbit annulus fibrosus cells and bone marrow-derived mesenchymal stem cells preliminary study co-culture.doc

 PAGE \* MERGEFORMAT 19 Rabbit annulus fibrosus cells and bone marrow-derived mesenchymal stem cells preliminary study co-culture Authors: Zhang Fuyong, Xiao-Tao Wu, Yun-Tao Wang, Bao Jun-Ping, Zhu Lei, Qiu Yun-Feng 【Abstract】 Objective: To investigate the bone marrow-derived mesenchymal stem cells (BMMSCs) and annulus fibrosus (AF) cells co-cultured on cell proliferation and the main effects of extracellular matrix, and further to determine BMMSCs transplantation on the prevention of disc degeneration and therapeutic effects. Methods: The rabbits were cultured BMMSCs and AF cells, will be two kinds of cells, the ratio of 1:1 mixing, using centrifuge tube culture technique for 3 weeks after the subjects, the general observation of parallel HE staining; the use of fluorescent dyes Hoechst33258 detect cell proliferation; through Real-time fluorescence PCR in type Ⅱ collagen and proteoglycan content were determined. Results: After 3 weeks in vitro, cell mass of the volume to reach the largest group of smear staining showed cells co-cultured with dense, cell proliferation significantly compared with the control group, real-time fluorescence PCR results showed that type Ⅱ collagen and proteoglycan mRNA expression increased. Conclusion: The three-dimensional culture in the centrifuge tube under the conditions, BMMSCs cells were cultured with AF can promote the proliferation, an increase of type Ⅱ collagen and proteoglycan content. Keywords: bone marrow mesenchymal stem cells; annulus fibrosus cells; bio-medical engineering; cell culture; rabbit Low back pain is a common orthopedic disease, a high incidence, 75% ~ 80% of people at some stage in their lives will be plagued by low back pain. Intervertebral disc degeneration leading to the most common cause of low back pain, clinical common surgical and conservative treatment although able to achieve a certain therapeutic effect, but not for the disc degeneration itself, the current treatment