Rabbit intervertebral degeneration of cartilage endplate instability Experimental Study.doc

 PAGE \* MERGEFORMAT 14 Rabbit intervertebral degeneration of cartilage endplate instability Experimental Study Of: Zhao, Huo Hongjun, Yang Xuejun, Zhong Xiu, Xing Wenhua [Abstract] [Objective] The study by electron microscope in the intervertebral instability cartilage endplate degeneration environment for the treatment of intervertebral disc degeneration provide new ideas and methods. [Method] 48 6 months old Japanese big ear rabbits, irrespective of gender, body weight (2.5 + -0.2 kg, were randomly grouped into control and experimental groups, control group 21 rats in the experimental group was 27, the first experimental group of rabbit fur cut off the lower back, diazepam injection 1.25 mg / kg, ketamine 0.02 g / kg, atropine 0.125 mg / kg in turn ear vein injection of anesthesia, prone on the operating table fixed with 1% povidone-iodine disinfection of the surgical field to the iliac crest level of intervertebral space (that is, L6, 7 as the center, taken from the middle of a 4 cm long longitudinal incision of skin and subcutaneous tissue, sharp separation, exposed spinous process, lamina and the upper and lower articular process, will be attached to the spine conflict, lamina and the whole part of the small joints of the muscles left, followed by removal of L6, 7, and interspinous ligament on the spine, bite than 6 under lumbar facet on both sides, resulting in intervertebral instability, wound with sterile normal saline , followed by stitching layers of tissue, after animals in a cage free. experimental group respectively after 2 months, 4 months, 6 months, drawn in the control group kept under the same conditions after 2 months, 4 months 6 months, drawn on the endplate cartilage endplate cartilage using transmission electron microscopy structure of an integrated determine the cartilage endplate degeneration. [results] can lead to instability of intervertebral intervertebral cartilage collagen fiber structure by the end plate orderly, arranged clo