Radiation damage in mouse bone marrow mesenchymal stem cells and osteogenic potential of hematopoietic stem cell niche of.doc

 PAGE \* MERGEFORMAT 18 Radiation damage in mouse bone marrow mesenchymal stem cells and osteogenic potential of hematopoietic stem cell niche of Of: Ma Jie, Shi Mingxia, Li Bingzong, Li Jing, Bai Chun room, Sun Hui, Chun-Hua Zhao [Abstract] Objective To observe the radiation on bone marrow mesenchymal stem cells (bone marrow mesenchymal stem cells, BMMSCs) in vitro osteogenic potential and in vivo bone marrow hematopoietic stem cells (hematologic stem cells, HSCs) niche. Methods isolated and cultured normal and accepted 4Gy mice at different time points after radiation BMMSCs, with alkaline phosphatase (alkaline phosphatase, ALP) staining and real-time quantitative RT PCR detection of osteoblast differentiation-related genes, identification BMMSCs osteogenic differentiation potential changes, and through bone tissue morphological and immunohistochemical method to detect in mice after radiation and spindle-shaped osteoblasts, neural cadherin expression in osteoblasts (spindle shaped N cadherin expressing osteoblasts, SNOs) number. Results Total body irradiation (total body irradiation, TBI) in mice early after the osteogenic potential of enhanced BMMSCs, but then decreased rapidly, TBI 28d mice after bone marrow osteoblasts and SNOs significantly reduce the number. Conclusion 4Gy long-term radiation injury in mice BMMSCs significant reduction in osteogenic potential in vivo niche HSCs was significantly reduced. [Keywords:] Objective To observe the radiation on bone marrow mesenchymal stem cells (bone marrow mesenchymal stem cells, BMMSCs) in vitro and in vivo osteogenic potential of bone marrow hematopoietic stem cells (hematologic stem cells, HSCs) niche. Methods isolated and cultured normal and accept 4Gy mice at different time points after radiation BMMSCs, with alkaline phosphatase (alkaline phosphatase, ALP) staining and real-time quantitative RT PCR detection of osteoblast differentiation-related genes, identification of osteoblast differentiatio