Recombinant human bone morphogenetic protein -7 on high glucose-induced mesangial cell transforming growth factor-1 and connective tissue growth factor expression.docVIP

 PAGE \* MERGEFORMAT 12 Recombinant human bone morphogenetic protein -7 on high glucose-induced mesangial cell transforming growth factor-1 and connective tissue growth factor expression OF: Du Yueguang with Fang-Fang Li Wan Hai [Abstract] Objective To study recombinant human Bone Morphogenetic Protein -7 (rhBMP-7) on high glucose-induced mesangial cell transforming growth factor-1 (TGF-1), connective tissue growth factor (CTGF) gene expression and extracellular matrix (ECM) component collagen type (Col ) and fibronectin (FN) secretion. Methods Cultured rat mesangial cells, is divided into four groups: normal control group (DMEM medium with 1 000 mg / L glucose), high glucose (DMEM medium containing 4 500 mg / L glucose), rhBMP-7 low-dose group (DMEM medium containing 4 500 mg / L glucose +50 nmol / L rhBMP-7 ), rhBMP -7 high dose group (DMEM medium containing 4 500 mg / L of glucose +100 nmol / L rhBMP-7). RT-PCR detection of TGF-1 and CTGF gene expression, ELISA was detected in the supernatant TGF1, Col , FN levels. results in high glucose mesangial cells TGF-1 and CTGF mRNA levels were significantly higher than the control group (P lt;0.01), TGF-1, Col and FN secretion increased, and the normal control group, the difference There were significant (P lt;0.05); and high glucose group, rhBMP-7 with different doses (50,100 ng / ml) TGF-1 and CTGF mRNA expression was significantly reduced supernatant TGF-1, Col , FN Secretion also significantly reduced, and the significant differences (P lt;0.05). Conclusion RhBMP-7 by inhibiting high glucose-induced mesangial cell TGF-1, CTGF gene expression and the secretion of ECM can play an anti-fibrosis effect. [Keywords:] rhBMP-7; transforming growth factor-1; connective tissue growth factor; extracellular matrix [Abstract] ObjectiveTo investigate the effect of rhBMP-7 on the expression oftransforming growth factor-1 (TGF-1), connective tissue growth factor (CTGF) and the extracellular matrix type collagen (