Refined safflower chitosan flocculation process of water extract.doc

 PAGE \* MERGEFORMAT 10 Refined safflower chitosan flocculation process of water extract Author: Chen Ying, Li Yongji, Cheng Shuyun, bradycardia 【Abstract】 Objective Optimization of chitosan refined safflower water extract process. Methods L9 (34) orthogonal design table, to HSYA A yield as an indicator, by high performance liquid chromatography content of chitosan refined safflower process optimization. The optimum results for the A3B2C1, will concentrate to the crude drug liquid: liquid = 1:6, chitosan dosage of 0.4 ml / g crude drug, storage time 6 h. Conclusion Chitosan flocculation to clarify the effect is good, economical and practical, can be used for water extract of refined safflower. Keywords: chitosan; safflower; high-performance liquid chromatography; orthogonal experiment Abstract: ObjectiveTo optimize refinement of Carthamus tinctorius L. by chitosan flocculation.MethodsApplying the L9 (34) of orthogonal design and high performance liquid chromatography (HPLC), with the yield rate of hydroxy safflower yellow A. as the index. ResultsThe optimized condition was A3B2C1, which was that medicinal herb: physic liquor = 1:6, the addition of chitosan was 0.4 ml / g medicinal herb, setting 6 hours.ConclusionChitosan flocculation method was effective and economic for the clarification and can be used in the refinement of Carthamus tinctorius L. Keywords:: Chitosan; Carthamus tinctorius L.; HPLC; Orthogonal design Naodesheng from 37, safflower, puerarin, Chuanxiong, Hawthorn 5 taste of Chinese herbal medicines, of which the Compositae safflower safflower Carthamus tinctorius L. The dried flowers, modern pharmacological studies have shown that the cardiovascular system has wide-ranging it a prominent role, which chalcone glycosides - HSYA A role for promoting blood circulation Huayu main active ingredients. Using the traditional refining process, need to consume a large amount of ethanol, the production costs are hi