Resveratrol induced apoptosis in primary rat adipocytes and its mechanism.docVIP

 PAGE \* MERGEFORMAT 18 Resveratrol induced apoptosis in primary rat adipocytes and its mechanism Of: Chen Si Fan, Zhou Niman, Zheng Lin, Ke Liangru, single Chi-ming, Feng Xiang [Abstract] [Objective] resveratrol (Res) on rat primary adipose cells and its mechanism. [Methods] cultured primary rat adipocytes, adding different doses of Res intervention, with Hoechst 33258 staining in the nuclear morphology was observed under fluorescence microscope; experimental observations with DNA Ladder DNA bands break; detected within each group and the cell culture medium of lactate dehydrogenase (LDH) were calculated leakage rate of LDH; by flow cytometry apoptosis; Western blot detection of related sites, including the silent information regulator factor 1 (Sirt1), cytochrome C (Cytochrome C), cysteine #8203;#8203;aspartase family (Caspase 9 and Caspase 3) protein expression. [ Results] Res intervention, a high degree of nuclear chromatin condensation, after electrophoresis multiple of 180 ~ 200 bp oligonucleotide fragments. cell LDH leakage rate increased in a dose - response relationship (P lt;0.05 or P lt;0.01) . Res role in the apoptosis rate increased significantly after a dose - response relationship (P lt;0.01). Sirt1, Cytochrome C, Caspase 9 activation and increased expression of Caspase 3, Cytochrome C release of cytoplasm increased. [Conclusion] Res promote apoptosis in primary fat cells, which may be by increasing the expression of Sirt1, through the mitochondrial apoptosis pathway, the role of Caspase 9 and Caspase 3. [Keywords:] resveratrol; apoptosis; silent information regulator 1; cytochrome C; cysteine #8203;#8203;aspartase Abstract: [Objective] To explore the effect and mechanism of resveratrol (Res) on apoptosis of rat primary adipocytes. [Methods] Rat primary adipocytes were cultured and treated with resveratrol in different dosages. Adipocytes were stained by Hoechst 33258, and cell morphological transformation was examined with fluorescen