RGS4 in HUEVCs apoptosis effect of dexamethasone.docVIP

 PAGE \* MERGEFORMAT 16 RGS4 in HUEVCs apoptosis effect of dexamethasone Of: Chen Xingyun, Zhao Yan, Li Ping, Xiong Yan, Liu Ping, Yang Nan, FORCES [Abstract] Objective RGS4 on normoxic and hypoxic culture conditions HUEVC apoptosis and the role of dexamethasone in which to explore the RGS4 in HUEVC cell apoptosis and the influence of dexamethasone treatment on . Methods O2/N2/CO2 simulated three-gas incubator culture conditions of normoxia and hypoxia HUEVC cells, RGS4 eukaryotic expression vector transfection HUEVC cells by flow cytometry (Annexin V FITC PI assay of apoptosis. Results normoxia RGS4 in transfected cells and dexamethasone treatment had no effect on apoptosis, cell apoptosis under hypoxic conditions increased (P lt;0.01), RGS4 could HUEVC after transfection apoptosis was significantly reduced (P lt;0.01), while dexamethasone and RGS4 apoptosis after treatment HUEVC transfected with RGS4 alone was no significant difference. Conclusion RGS4 does not affect the normal oxygen conditions HUEVC apoptosis, but significantly inhibited the hypoxia conditions HUEVC apoptosis, dexamethasone suppression of apoptosis may be related to RGS4 on or through the same cell signaling pathway of apoptosis under hypoxic conditions. [Keywords:] dexamethasone RGS4 apoptosis Abstract Objective To study the role of RGS4 in apoptosis of HUEVCs in normoxia or hypoxia and the effect of dexamethasone on it.Methods HUEVCs were cultured in the simulated normoxia or hypoxia of O2/N2/CO2 cell incubator and were transfected with the eukaryotic expression vector of RGS4; the flow cytometer (Annexin-V-FITC-PI) was applied to detect its apoptosis. Results The transfection of RGS4 and treatment of dexamethasone had no obvious effect on the apoptosis in normoxia while the apoptosis of HUEVCs increased in hypoxia (P lt;0.01); the apoptosis of HUEVCs decreased significantly after RGS4 transfection (P lt;0.01); the apoptosis of HUEVCs treated with dexamethasone and with RGS4