Research progress of E. coli sRNA.docVIP

 PAGE \* MERGEFORMAT 13 Research progress of E. coli sRNA Study: More Li Hongtao Wang Hong Li [Keywords:] Escherichia coli; RNA / no translation; Research Methods sRNAs that small RNAs or non-coding RNAs (noncoding RNAs) are a class of tens to hundreds of nucleotides in between, the implementation of various functions of the non-coding RNA [1]. sRNA molecule was originally discovered in bacteria , now known widely in bacteria from prokaryotes to higher mammals such as a variety of living organisms, mainly from the regulation of gene expression. In this paper, the prokaryotes E. coli, for example, the discovery of its sRNA, function , the possible mechanism and explore new methods and Hbq protein sRNA sRNA perform functions in the role are reviewed. 1 sRNA discovery In 1967, all by E. coli RNA were labeled in vivo, in the polyacrylamide gel to detect a large number of new RNA molecules, so that the first E. coli sRNA 6SRNA [2] was found, followed by have been found 11 sRNA, which RprA, MicF, DicF, and DsrA as a gene fragment was found; GcvB and OxyS exploration genes are discovered accidentally, and Spot42, 10Sa (tmRNA) and 10Sb other is marked by phosphorylation after all the RNA found in the (now known as M1, is the RNA component P RNA enzymes) [37]. but their physiological function until 30 years before being elucidated. prokaryotic sRNA length is generally 50 ~ 250nt between the maturity longer than eukaryotic miRNA, which are not normally in the cell translation, but as an important regulatory factor involved in transcription and translation of prokaryotic gene regulation, and RNA stability, the process of maturation and processing, and other . 2 method of identifying sRNA Since sRNA probe by experimental or traditional sequence analysis methods is difficult to detect, so even though we know that these RNA molecules on the physiological functions of organisms plays an important role, but in practice many of the sRNA molecules are in the