Rg1 notoginseng saponins on hemorrhagic shock in rats of intestinal epithelial cells of mitochondrial function.docVIP

 PAGE \* MERGEFORMAT 17 Rg1 notoginseng saponins on hemorrhagic shock in rats of intestinal epithelial cells of mitochondrial function OF: LEE Wai Man, Fangchuan Qin, Lu Songmin Liu Jiancang, Guosu Qing, Cheng Feng, Zheng-Guo Wang [Abstract] Objective To observe notoginseng saponins on hemorrhagic shock in rats Rg1 in intestinal epithelial cells cytochrome oxidase (COX activity and membrane potential. Methods 24 healthy Wistar rats were randomly divided into 4 groups: normal the control group (N), 5 hours group hemorrhagic shock (HS), a simple recovery of the control group (RE), the recovery plus Rg1 group (Rg), n = 6. spectrophotometric detection by fluorescence of mitochondrial membrane potential; by UV COX activity by spectrophotometry. 5 hours of hemorrhagic shock results in intestinal epithelial cells in rats reduced mitochondrial uptake Rhodamine123 that membrane potential was significantly lower (P lt;0.01, simple resuscitation membrane potential slightly increased the recovery of mitochondrial membrane plus Rg1 group potential of significantly improved, restored to normal levels. hemorrhagic shock 5 hours mitochondria in intestinal epithelial cells significantly decreased COX activity, the simple recovery of its activity is still low. resuscitation plus Rg1 COX activity than the treatment group significantly increased compared shock and significantly higher than the recovery group. Conclusion recovery plus Rg1 can significantly increase the hemorrhagic shock in rats intestinal epithelial cells in COX activity and mitochondrial membrane potential. Rg1 increased COX enzyme activity may increase the yield and quality related. [Keywords:] hemorrhagic shock, intestinal epithelial cells, notoginseng saponins Rg1, cytochrome oxidase, mitochondrial membrane potential [Abstract] Objective To study the effects of Rg1 isolated from panax notoginseng saponins (PNS) on mitochondrial cytochrome oxidase (COX) and membrane potential of small intestinal ep