Rhizoma Imperatae Optimization of ultrasonic extraction of polysaccharide.docVIP

 PAGE \* MERGEFORMAT 10 Rhizoma Imperatae Optimization of ultrasonic extraction of polysaccharide 【Abstract】 Objective To determine the optimum ultrasonic extraction conditions of Rhizoma Imperatae polysaccharide extract Rhizoma Imperatae polysaccharide, for Rhizoma Imperatae research, development and utilization provide a theoretical basis. Methods Rhizoma Imperatae polysaccharide content of the indexes, using phenol - sulfuric acid assay method using the orthogonal experimental method to determine the best ultrasound Rhizoma Imperatae polysaccharide extraction conditions. The results of extraction times and the solid-liquid ratio on the extraction rate of polysaccharides Rhizoma Imperatae play a major role. The optimum ultrasonic extraction as follows: extraction temperature 80 ℃, with 20 times the amount of water, ultrasonic extraction 3 h, extracted three times, Rhizoma Imperatae polysaccharides yield of 6.33%. Conclusion determined Rhizoma Imperatae optimal ultrasonic extraction process of polysaccharide. 【Key Words】 Rhizoma Imperatae Rhizoma Imperatae polysaccharides extracted orthogonal ultrasonic phenol - sulfuric acid method Rhizoma Imperatae only contained in the “Shen Nong’s Herbal Classic”, formerly known as Chine, for grasses Imperata Imperata cylindrical (L.) P. Beauv var.major CEHubb.’s Roots [1], Sweet, cold, with the cooling blood to stop bleeding , heat-diuretic achievements. For the treatment of hematemesis, gonorrhea, negative urine, edema, and jaundice. Studies have shown that the active ingredient triterpenoids and sugar accounted for 18.8% of the total chemical composition [2]. Eglinton and Natory team has done a great deal of research to its constituents, has isolated elements of the Arundo donax, Imperata cylindrical element, fern-ol, and other triterpenoids. Rhizoma Imperatae aqueous crude extract by gel filtration and anion-exchange chromatography for separation of a series of high molecular weight polysacchari