Ricinine and structural analysis of the extraction and purification_0.docVIP

 PAGE \* MERGEFORMAT 10 Ricinine and structural analysis of the extraction and purification [Keywords:] Structure 1 Introduction Castor castor-oil base is obtained by an important ingredient in castor meal, castor present in leaves and stems and seeds, cotyledons in the bud, especially high content of castor cake without Detoxification meal content of up to 0.2% to 0.4%. Ricinine pure white needle-like or prism-like crystalline alkaloid, mp 201 ~ 205 ℃, at 170 ~ 180 ℃, 2.667 kPa sublimation. Ricinine soluble in hot water and hot chloroform, hot ethanol in a certain solubility, is a neutral alkaloid, has a natural activity, aqueous solution is neutral, not easily form salts with acids. The alkaline solution enables high-manganese oxide potassium reduction, while generating hydrogen cyanide. because molecules containing cyano, toxic, can cause nausea, vomiting, severe respiratory failure and even death. At present, research ricinine study focused on the feed. Extraction Process The extraction rate of about 0.15%, the experimental extraction of castor meal ricinine conditions were optimized, the extraction rate to 0.35%, and the structural analysis to obtain a clear spectrum. 2 Experimental part 2.1 Apparatus, reagents and materials DK 8D tank type electric thermostat, Soxhlet extractor, RE52 98 rotary evaporator, LC / MSD TRAP SL mass spectrometer (U.S. Aligent company, RX400 NMR instrument. Chloroform, ethanol, methanol (HPLC grade, bismuth iodide K reagent, other reagents were of analytical grade. castor cake by the city of Tonghua Castor Tongliao Chemical Co., Ltd. to provide. Ricinine standard products for the laboratory-made (verified by the HPLC purity of 99.4%. 2.2 Sample Preparation and Purification Ricinine extraction process: castor meal → → filter concentrated aqueous extract → → → chloroform extraction ethanol reflux recrystallization → Ricinine needle-like crystals. Ricinine extracts will be condensed into extract and placed i