Ricinus communis root extract on HepG2.2.15HBsAg and HBeAg expression and antibacterial activity of.docVIP

 PAGE \* MERGEFORMAT 14 Ricinus communis root extract on HepG2.2.15HBsAg and HBeAg expression and antibacterial activity of Of: Tangzu years, Xu Yajuan, Feng Mei, Li Yongwen [Abstract] Objective To investigate the root extract of castor HepG2.2.15 cell line expression of HBsAg and HBeAg and anti-bacterial effect. Methods HepG2.2.15 cell culture system for detection of Ricinus communis root extract expression of HBsAg and HBeAg; with plate method, serial dilution of extracts determined the minimum inhibitory concentration. The results of two alcohol root extract of castor HepG2.2.15 cells were incubated for 72 h, inhibited the expression of HBsAg and HBeAg (with the normal control group P lt;0.05 or P lt;0.01), its inhibitory effect was concentration dependent, with 50% ethanol extract at 200 mg / L and 100 mg / L inhibited the expression of HBsAg were 100% and 67.2%. Ricinus communis root extract of the minimum inhibitory concentration (MIC), with 50% ethanol extract of S. aureus 3 and 4, Staphylococcus epidermidis, Escherichia coli strain 2 strong inhibitory (MIC were 19.5 μg / ml). followed by gold 2 MRSA strains, 3 E. coli strains of influenza A and B streptococci, Streptococcus pyogenes, Streptococcus pneumoniae, Pseudomonas aeruginosa (MIC were 39.1 μg / ml), and 95% ethanol extract weak inhibitory effect. Conclusion root ethanol extract of castor with in vitro anti-hepatitis B virus and antibacterial effects. [Keywords:] castor root extract; HepG 2.2.15 cell line; HBsAg; HBeAg; antibacterial activity Castor Ricinus communis L., the Euphorbiaceae plant, also known as bovine Grate grass, red castor, Le vegetables, straw, flax. According to lt;lt;Compendium of Materia Medicagt;gt; records castor bean, smells sweet, acrid, flat, a small drug , the head of water Wei. to study twenty service of water, spit evil foam, add to the thirty three days one, recover from illness is just. and the main wind Deficiency heat, itching sores body edema, body Zhu