RP-HPLC method for simultaneous determination of Mailuoning injection of 6 of the Content of phenolic acids.doc

 PAGE \* MERGEFORMAT 11 RP-HPLC method for simultaneous determination of Mailuoning injection of 6 of the Content of phenolic acids Of: Zhang Hui, Li Xiang, ZHOU Hong-yan, Xu Xiangyang, Chen Weiqi [Abstract] Objective To develop a HPLC method for simultaneous determination of Mailuoning injection of 6 methods of phenolic acids content. Methods Kromasil-C18 column (4.6 mm × 250 mm, 5 μm), mobile phase: acetonitrile - 0.1% phosphoric acid, gradient elution, flow rate: 1.0 mL / min, UV detection wavelength: 327 nm, column temperature: 25 ℃, and the injection volume: 20 μL. the result of new chlorogenic acid, chlorogenic acid, chlorogenic acid hidden, caffeic acid, 3,5 - caffeoylquinic acid, 3,4 - caffeoylquinic acid linear range of 0.010 90 ~ 0.544 8 μg (r = 0.999 9), 0.024 06 ~ 1.203 2 μg ( r = 1), 0.011 06 ~ 0.552 8 μg (r = 1), 0.015 94 ~ 0.796 8 μg (r = 1), 0.009 104 ~ 0.455 2 μg (r = 1), 0.010 37 ~ 0.518 4 μg (r = 1), the recoveries were 101.06%, 100.22%, 101.54%, 99.42%, 100.21%, 101.65%. Conclusion The method is sensitive and simple, accurate and reliable, can be used as the new Mailuoning injection of chlorogenic acid, chlorogenic acid, hidden chlorogenic acid, caffeic acid, 3,5 - caffeoylquinic acid, 3,4 - caffeoylquinic acid method for simultaneous determination of [Keywords] context of injection, the new chlorogenic acid, chlorogenic acid, hidden chlorogenic acid, caffeic acid, 3,5 - caffeoylquinic acid, 3,4 - caffeoylquinic acid, RP high liquid chromatography Abstract: Objective To establish a method for determination the contents of six phendic acids in Mailuoning injection by HPLC at same time. Methods Separation was performed on a Kromasil-C18 (4.6 mm × 250 mm, 5 μm) column with mobile phase consisting of 0.1 % phosphoric acid-water and Acetonitrile with gradient elute at the flow rate of 1 mL / min. The UV detection wavelength was set at 327 nm and the column temperature was set at 25 ℃. The sample size was 20 μL. Results The standard cu