RP-HPLC method for simultaneous determination of ursolic acid and verbena in the content of oleanolic acid.doc

 PAGE \* MERGEFORMAT 10 RP-HPLC method for simultaneous determination of ursolic acid and verbena in the content of oleanolic acid [Abstract] Objective To establish a RP-HPLC determination of ursolic acid and verbena oleanolic acid content. Method column of Nucleosil C18 column (4.6 mm * 250 mm, 5 μ m), mobile phase was methanol - water (88:12, V / V), flow rate of 0.8 ml / min, detection wavelength was 210 nm, column temperature 25 ℃ . Results Ursolic acid within 0.458 ~ 4.109 μ g showed good linear relationship (r = 0.999 7), the average recovery was 98.1%, RSD = 1.1%; oleanolic acid in the 0.156 ~ 1.389 μ g was well within the scope linear relationship (r = 0.999 6), the average recovery was 101.7%, RSD = 1.7%. CONCLUSION This method is simple, rapid, accurate and reliable, and can be used as quality control of medicinal herbs verbena. [Keywords:] Verbena; Ursolic acid; oleanolic acid; RP-HPLC Abstract: ObjectiveTo establish a method for determining ursolic acid and oleanolic acid in Verbena officinalis L. by high performance liquid chromatography. MethodsThe separation was carried out on Nucleosil C18 column (4.6 mm * 250 mm, 5 μ m) with methanol-water (88: 12, V / V) as the mobile phase.The flow rate was 0.8 ml / min and the detection wavelength was 210nm. The column was used at 25 ℃ . ResultsUrsolic acid showed a good linear relationship in the range of 0.458 ~ 4.109 μ g ( r = 0.999 7) and the average recovery was 98.1%. RSD was 1.1%. Oleanolic acid showed a good linear relationship in the range of 0.156 ~ 1.389μ g (r = 0.999 6) and the average recovery was 101.7%. RSD was 1.7 %. ConclusionThe method is rapid, simple, accurate and reliable and can be used for the quality control of Verbena officinalis L.. Keywords:: Verbena officinalis L.; Ursolic acid; Oleanolic acid; RP-HPLC Plants for the Verbenaceae Verbenaceae Verbenaceae Verbena officinalis L. aerial parts of the dry, also known as iron whip, whip of malaria, Chenopodium ambrosioides. Verbena