RP-HPLC on the skin disease of matrine in lotion.doc

 PAGE \* MERGEFORMAT 8 RP-HPLC on the skin disease of matrine in lotion [Abstract]: Objective To establish skin diseases lotion Determination of matrine. Methods HPLC, Inertsil NH2 column (250 mm × 4.6 mm, 5 μm), acetonitrile - ethanol -3% phosphoric acid (83:8.5:8.5) as the mobile phase, flow rate 1.0 mL / min, detection wavelength was 220 nm. Results of matrine in the range of 0.039 ~ 1.560 μg good linear relationship (r = 0.999 7), the average recovery was 97.8%, RSD = 1.1%. Conclusion The method is simple, rapid, accurate, and can be used for the preparation of quality control. [Keywords:] skin disease lotion HPLC matrine Abstract: Objective To establish a method for determination of matrine in Fuji Lotion. Methods An Inertsil NH2 column (250 mm × 4.6 mm, 5 μm) was used with acetonitrile-ethanol absolute-3% phosphoric acid (83:8.5:8.5) as mobile phase, the flow rate was 1.0 mL / min, and the UV detection wavelength was set at 220 nm. Results The calibration curve of matrine was linear in range of 0.039 ~ 1.560 μg, r = 0.999 7. The average recovery was 97.8% with RSD of 1.1% (n = 6). Conclusion The method is simple, quick and accurate. It can be used for the quality control of Fuji Lotion. Keywords:: Fuji Lotion, matrine, RP-HPLC Skin disease lotion contained in the Ministry of Health received ministerial standard “prescription of Chinese medicine formulationsgt;gt; fifteenth book [1], by flavescens, one hundred, pepper and other herbs composed of 6, with insecticide detoxification, only itch convergence, stasis of power. in the standard, only the simple identification and inspection items, and its determination method not reported. In order to effectively control the drug quality, this study established a skin disease in the lotion herbs Sophora Determination of matrine, using high performance liquid chromatography preparation of matrine were determined, the method is simple, rapid, accurate, and lotion for skin diseases provide the basis for