RPHPLC determination of puerarin Xinkeshu the content of the film.docVIP

 PAGE \* MERGEFORMAT 12 RPHPLC determination of puerarin Xinkeshu the content of the film [Abstract] [Objective] To establish Xinkeshu Tablets Puerarin HPLC Assay. [Method] high-performance liquid chromatography; chromatographic conditions were: ZORBAX Eclipse XDB  C18, column temperature was 25 Celsius; mobile phase: methanol - water - phosphoric acid (23:77:0.5); flow rate: 1.0ml.min-1; detection wavelength ; 250nm. [Results] puerarin in the 25ng ~ 250ng range a good linear relationship, the regression equation Y = 4663.9774X (r = 0.9998), the average recovery was 102.34%, RSD = 2.7197% (n = 5). [Conclusion] This method is stable, reliable, simple, rapid and reproducible and can be used Xinkeshu tablets puerarin quality control. [Keywords:] Xinkeshu piece of puerarin HPLC Abstract: [Objective] Set up measurement method for measuring puerarin in Xinkeshu tablets with HPLC. [Method] HPLC, chromatogram: ZORBAX Eclipse XDB  C18; column temperature: 25C degree; flow phase: methanol  water  phosphor (23:77: 0.5); flow speed: 1.0ml * min-1; tested wave length: 250nm. [Result] In the range of 25ng ~ 250ng, puerarin has good linear relationship, with regress equation: Y = 4663.9774X (r = 0.9998), the average pushing-sample reclaim rate is 102.34%, RSD = 2.7197% (n = 5) [Conclusion] The method is stable, reliable, easy to operate, quick, with good repeat, can be used in quality control of puerarin in Xinkeshu tablets. Keywords:: Xinkeshu tablets; puerarin; HPLC Xinkeshu film is from the hawthorn, puerarin, Salvia, 37, costas 5 refined taste of Chinese medicine made of pure Chinese medicine, has increased hypoxia tolerance, anti-myocardial ischemia, lipid-lowering, antihypertensive, anti-arrhythmia, promote fibrinolysis, anti-blood viscosity, blood circulation, relieve pain and other effects of qi, mainly for Qi stagnation blood stasis type of chest tightness caused by coronary heart disease, angina, high blood pressure, dizziness, headache, neck pain an