Salmonella pathogenicity pig tetB Tetracycline resistance genes detected in PCR.doc

 PAGE \* MERGEFORMAT 17 Salmonella pathogenicity pig tetB Tetracycline resistance genes detected in PCR Author: Liu Wei-Hong Xu Yindi Guo Aizhen Guai Qing Liu Fa Chen HC [Keywords:], Salmonella;,,,, tetracycline resistance gene;,,, tetB;,,, PCR;,,, pig Abstract: Objective To determine the pathogenicity of clinical isolates of Salmonella in swine tetracycline resistance genes, tetracycline resistance genes to determine the type of Salmonella. Methods Determination of KB isolates to tetracycline and other antibiotics of 21 cases, with PCR used to detect tetracycline resistance gene tetA, tetB, tetC, tetD, tetE, tetG and tetK, and amplified products were sequenced. The results of all clinical isolates resistant to tetracycline, 33 Salmonella has 29 specific fragments amplified tetB, gene sequence homology with the reference strains of 99%, tetB detection and drug susceptibility testing positive consistent rate was 879 %. Conclusion tetB of PCR detection of tetracycline resistance have a high specificity, tetB genes in this experiment is to determine tetracycline-resistant clinical isolates of the major genes for tetracycline resistance provides the basis for molecular epidemiology surveillance. Keywords:: Salmonella; tetracycline resistance gene; tetB; PCR; pigs PCR amplification of tetB gene for pathogenic Salmonella isolated from diseased pigs ABSTRACT Objective To detect the tetracycline resistance genes for 33 Salmonella isolates from diseased pigs and define the tetracycline resistance determinants. Methods The resistance of Salmonella isolates were detected by drug susceptibility tests with KirbyBauer (KB) diffusion method. PCR was designed to detect tetA, tetB , tetC, tetD, tetE, tetG and tetK. The amplified products were sequenced and alignment analysis was performed. Results The detection of tetracyclineresistance of 33 Salmonella isolates showed the resistance rate was 100%, but only the tetB was amplified from 29 of 33 isolates . The gene fragment