Salvia malignant obstructive jaundice in rats when the role of apoptosis in tumor cells.docVIP

 PAGE \* MERGEFORMAT 2 Salvia malignant obstructive jaundice in rats when the role of apoptosis in tumor cells [Keywords:] Cholestasis; invasion and metastasis; proliferating cell nuclear antigen; Salvia; apoptosis index [Abstract] AIM: To establish malignant biliary obstruction models in SD rats, and to study the effects of Danshen injection on the growth of hepatocellular carcinoma, morphological change and PCNA expression and apoptosis index in carcinoma and pericarcinoma tissues. METHODS: ① Malignant biliary obstruction model in SD rats was made by vaccination of transplanted tumor in liver nearby the porta hepatis with Walker256 hepatocarcinoma cell line, which infiltrated proximal common bile duct to cause its narrowness and obstruction. ② The SD rats were divided into 4 groups that were treated ip by 0.9% normal saline (NS) (n = 16), by inosine vitamin C (n = 26), by Danshen (n = 34) and by 5FU (n = 30) respectively. ③ The diameter of transplanted tumor, morphological change and the expression of PCNA and apoptosis index in carcinoma and pericarcinoma tissues were observed. ④ Statistical analysis was made in the experimental data. RESULTS: In Danshen group as compared with NS group and inosine vitamin C group, the average size of hepatocarcinoma was reduced significantly, the tumor inhibitory rate and the metastasisinhibitory rate were higher obviously (P