Scorpion Buthus epileptic mouse cerebral cortex extract on NMDA receptors and regulation of GABAA receptor.docVIP

 PAGE \* MERGEFORMAT 3 Scorpion Buthus epileptic mouse cerebral cortex extract on NMDA receptors and regulation of GABAA receptor [Keywords:] Buthus scorpion extract;,, GABAA receptors;,, NMDA receptor;,, epilepsy Abstract: Objective To study the BmKE against pentylenetetrazol-induced epileptic seizure and its effect on NMDAR and GABAAR mouse cerebral cortex in vitro. Methods pentylenetetrazol (PTZ) induced epileptic model mice, sodium valproate (VAP, positive control) treatment group and BmKE low (L), Chinese (M), High (H) dose of epileptic seizures in mice treated The incubation period, the application 3H isotope marked MK-801, respectively, and Mucimol, detection of mouse cerebral cortex NMDAR and GABAAR binding activity. Results BmKE (M) could significantly prolong the onset of PTZ-induced seizures in mice the incubation period (P amp;lt;0.05), BmKE (L) plays a minor role, BmKE (H) treated epileptic seizures in mice to extend the value of the incubation period than BmKE (M) treatment of were lower. Significantly increased PTZ-induced seizures in mice cerebral cortex of the NMDAR binding activity (P amp;lt;0.05), BmKE (L) treated mouse cortical NMDAR binding activity decreased, but there was no significant difference, BmKE (M) and BmKE (H) seizures were significantly reduced NMDAR binding activity in mouse cerebral cortex (P amp;lt;0.001). BmKE (L) and BmKE (H) significantly increased GABAAR binding activity (P amp;lt;0.05), BmKE (M) was significantly increased GABAAR binding activity (P amp;lt;0.001). Conclusions can be effective treatment of moderate BmKE pentylenetetrazol induced seizures, significantly prolong the incubation period of epileptic seizures in mice, BmKE antiepileptic mechanism of action, may be related to inhibit NMDAR-binding activity, activation GABAAR combination of activity. Keywords:: scorpion extract; GABAA receptor; NMDA receptor; epilepsy The Modulation of Buthus martensic Karch Extract (BmKE) on Cerebral Cortex NMDA Receptor an