Selenium combined effects of lead on primary cultured hippocampal neurons influence.docVIP

 PAGE \* MERGEFORMAT 9 Selenium combined effects of lead on primary cultured hippocampal neurons influence [Keywords:] Lead; selenium; hippocampus; neuron; neurite Abstract: Objective To observe the lead and selenium on the primary cultured hippocampal neurons the growth and survival of the impact study of selenium on lead inhibition of nerve cell growth antagonism. Methods Wistar rats with neonatal hippocampal neurons in primary serum-free culture technology, through PbCl2, Na2SeO3 separately or in combination to observe nerve cell growth and survival. Results 10-5mol / L Pb significantly inhibited the primary cultured hippocampal neurons neurite growth, neuronal survival rate; separate selenium (2 * 10-6mol / L) had significantly promote neurite growth in hippocampal neurons the role of, in particular, In the early growth of neuronal processes; but the survival rate of hippocampal neurons was not obvious. Of lead and selenium, when co-incubated to develop an early lead on selenium can antagonize the inhibition of neuronal processes extend, but with the culture time extended, this antagonism disappeared; from neuron survival point of view, in the experimental doses, no selenium can antagonize the lead on neuronal survival in vivo. Conclusion Lead can inhibit the growth and survival of neurons toxic dose of selenium in this experiment in cell culture can promote neuronal processes early stage of growth and antagonism of lead on neuronal growth and survival in vivo. Keywords: lead; selenium; hippocampus; neuron; neurite Effect of lead and selenium on primary cultured hippocampal neurons. Abstract: Objective To study the effect of lead and selenium on the growth and survival of primary cultured hippocampal neurons.Methods The primary culture of hippocampal neurons of newborn Wistar rats with serumfree medium was established.After cocultured with PbCl2 and Na2SeO3 respectively and simultaneously, the growth of neurites and survival of neurons were tested.Resu