Table epigallocatechin gallate-induced apoptosis in human gastric cancer BGC823.docVIP

 PAGE \* MERGEFORMAT 22 Table epigallocatechin gallate-induced apoptosis in human gastric cancer BGC823 Author: Zou Shao-na, Wen Ling, Xiao-Ping Liu, Xiao-Yan Chen, Wang Qiang, LUO Zhao-yang Abstract [Objective] To observe the table epigallocatechin gallate (EGCG)-induced apoptosis in human gastric cancer BGC823 biological effects and molecular mechanism. [Methods] MTT assay EGCG on cell growth BGC823; by morphological observation, flow cytometry and DNA agarose gel electrophoresis BGC823 cell apoptosis induced by EGCG. Western blot to detect EGCG treatment BGC823 cell NF-κB (p65), Bcl-2, Bax, and Caspase-3 protein expression. [Results] EGCG significantly inhibited the growth of BGC823 cells, showing dose-dependent. EGCG treatment, light microscope, cells were typical morphological changes of apoptosis; flow cytometry analysis showed that after 48h of different concentrations of EGCG treatment, sub-G1 phase cells increased gradually; EGCG (80μg/ml) the role of 48,72 h later, DNA gel electrophoresis typical ladder; Western blot showed that 48h after EGCG treatment, NF-κB (p65), and decreased expression of Bcl-2 protein, Bax, and Caspase-3 protein expression increased. [Conclusion] EGCG can induce apoptosis in human gastric cancer BGC823, whose mechanism may be related to inhibiting NF-κB activation, resulting in Bax/Bcl-2 ratio increase, the promotion of Caspase-3 activation. Keywords: stomach neoplasms The main component of tea polyphenols (tea polyphenols) with anti-tumor effect. Polyphenols by several structurally similar compounds, the composition of polyphenols, including epigallocatechin table gallate (epigallocatechin-3-gallate, EGCG), epigallocatechin table (epigallocatechin, EGC), epicatechin gallate (epicatechin-3-gallate, ECG) and epicatechin (epicatechin, EC) and so on. In these types of monomers, EGCG content of the most abundant, the largest value [1]. Apoptosis regulation disorders leading to tumor formation