Zhibai Dihuang Wan on leptin-induced model of idiopathic precocious puberty in mice.docVIP

 PAGE \* MERGEFORMAT 18 Zhibai Dihuang Wan on leptin-induced model of idiopathic precocious puberty in mice Author: Liu Mengyuan, Wen Xu, Xiao Liuying, Han-chao [Abstract] [Objective] To observe Zhibai Dihuang Wan on serum leptin (Leptin)-induced idiopathic central precocious puberty (ICPP) model mice. [Methods] selected 18-day-old BALB / c female mice were randomly divided into six groups: normal control group, ICPP model group, Zhibai Dihuang Wan high-and low-dose group (dose, respectively 101.4,50.7 g * kg-1 * d-1), megestrol acetate in the control group (dose of 3.9mg * kg-1 * d-1), gonadotropin-releasing hormone analogue (GnRH  A) group (initial dose of 1 573.5μ g/kg, No. 2,3 doses were 1 180 μ g / kg, every other day 1; addition to the normal control group, other groups were injected intraperitoneally Leptin (20mg/kg) replication model; test mice in each group the number of vaginal opening, body mass , uterus, ovary mass and serum luteinizing hormone (LH), estradiol (E2) levels, and calculate the index of uterus and ovaries. [Results] Zhibai Dihuang Wan modeling high-dose group in the first 4 to 6 days of leptin by the To inhibit the vaginal opening ahead of schedule (all P amp;lt;0.05 or P amp;lt;0.01); Zhibai Dihuang Wan low-dose group of mice the uterus can reduce the quality of Leptin induced inhibition of uterine and ovarian index index increased (all P amp;lt;0.05 or P amp;lt;0.01); Zhibai Dihuang Wan high-and low-dose group of mice can reduce the LH levels (all P amp;lt;0.01), but mice of body weight and serum E2 level had no significant effect (all Pamp;gt; 0.05 ). [Conclusion] Zhibai Dihuang Wan ICPP role in the treatment may be related to able to inhibit the hypothalamus - pituitary - gonadal axis ahead of the launch. [Keywords:] Zhibai Dihuang Wan / pharmacol; sexual precocity / TCD therapy; Luteinizing Hormone / blood; Estradiol / blood; disease models, animal; mice Precocious puberty is a growth and developmental abnormalities, show