β-catenin in leukemia cell lines abnormal localization.docVIP

 PAGE \* MERGEFORMAT 20 β-catenin in leukemia cell lines abnormal localization Abstract β-catenin Wnt signaling pathway is a key signaling son, its location in the cell caused by the abnormal transcription of downstream target genes is a variety of solid tumors occurred in one of the reasons. Because adhesion of hematopoietic cells lack the typical connections (adherens junction, AJ), and therefore in the blood of β-catenin expression and location of the tumor is no more research. In this study four kinds of common leukemia cell lines (Daudi, Jurkat, K562 and Thp-1) in β-catenin protein localization and β-catenin gene mRNA expression levels to understand whether there are tumors of blood β-catenin Fibroin abnormal positioning in order to discover a new mechanism of leukemia, in order to search for new therapeutic targets for specific clues provided. Was detected by immunocytochemistry of β-catenin in leukemia cell lines in the position, with real-time quantitative RT-PCR Determination of β-catenin mRNA expression level. The results showed that: four kinds of leukemia cell lines, there are two (Jurkat, Thp-1) the existence of β-catenin abnormal position, and the expression of β-catenin mRNA increased, but in Jurkat and Thp-1 cells of β-catenin mRNA below the Daudi and K562 cells. These four kinds of leukemia cell lines in the β-catenin gene mRNA expression levels had no correlation with its unusual location. Conclusion: β-catenin may be involved in abnormal positioning the occurrence of some of the blood cancer, causing abnormal β-catenin targeting mechanism may not occur at the transcriptional level. Keywords: β-catenin in leukemia cell lines Daudi K562 Jurkat Thp-1 Aberrant Localization of β-catenin in Leukemia Cell Lines Abstractβ-catenin plays a central role in Wnt signaling pathway. The aberrant localization of β-catenin in nucleus causes the transcription of down-stream target genes, which is the pathogenesy of some solid tumour