持续低温灌注对大鼠移植肝脏的保护作用.pdfVIP

中华肝坦外科盖曹志Zω8 年 10 月第 1. t墨第 10 期 Chin J H.patobili.ary Surg._Ü回~r 2008. Vol. 14. No.10 , 711 · 实验研究 · 持续低温灌注对大鼠移植肝脏的保护作用 朱健康县旭生王可新 草叶斌朱民刻板 E摘要】 目的 探讨离体供肝待续低温灌注对大鼠移植肝脏的保护作用. 方法 60 只 Wistar 大鼠随机分为3 组，但手术组 12 只.离体供肝造行待续性冷渥注组和常规汾保移组各 24 只.供肝保 存时间5 h . t!!手术组及后两室里受体分别于水后 24h留取血液、肝组织.并记24h平均阻汁流量. 各 组分别检测肝功能，测定跑汁中 γ-谷氨酸串串肤曾(G;T) 活性及萄萄糖(Clu) 含量. lf组织行常规病 理学榆聋.筋果 持续低温海泣组和常规汾保存级血清肝功能指标均高于假手术组( P0.05). 而 传续低温灌注忽较常规冷保存组低(P0.05). 待续低温灌注组平均每小时DI!汁流量明显高于冷保 存舰(P0.05) ， 持续低温攘注组瓶汁中 CCT 活位和 Glu 含最明显低于常规冷保存组(P0.05). 篇论 ...体供肝持续低渥灌注对大鼠移榄后肝功能有明显的保护作用. E关键词】 肝移植， 离体供肝z 低li灌注， 大鼠 ProtK1i昭effect 01 回回tin田圃 bYJ刚Mrmk 隙rfu.幅 幅阳晒planted U. er tn n lJ Z H U lian-Itang . llANGXu-纳Ln8. WANG KLxin . etal. Li时r TransþÚlrtUltion untLr. Qilu H osþital. Sh4ndong Universit;y. 1 inan 2500 12. P. R. China (AblItnlCt) 例如tin To explore Ihe prolective eHect of ∞ntlnωus hypolhermic perfusion on donor liver. Met阳炮 Sixty Wistar rats were random)y divided into the conlrol group (sham operalion group. n = 1 幻. donor conlinuous hypothermic reperfusion group (n = 24) 8nd 5t81ic cold slorage group (n=24). The donor keeping lime was 5 h. The samples of blood and hepalÎc tiSS IJe were col- lecled 24 h 811.r liver transplantation. Meanwhile . the bile f10w during the 24 h was recorded. 民甜ults Compared with Ihe ∞ntrol group. liver function8 1p8rameters were higher in othcr IWO groups (P o. 05). It was significantly lower in the conlÎnuous hypothermic reperfusion group than in Ihe static cold slorage group (PO. 05). Meanwhile. Ihe bile f10w in the continuous hYPOlhermic reperfusion group W8S mor. lhan lhat in the static cold slorage group (P O. 05) . While γ-GGT and the Glu∞5e (Glu) in the bile of the ∞ntinuous hypothermic reperfusioo group were lower thaD those in Ihe 归lic ∞Id atorage group (PO. 05). C倒时回制国 Continωωbypo也e