miR-218靶向Wnt2B抑制卵巢癌细胞转移侵袭功能.pdfVIP

Vo l. 42 No.6 P.656 华中科技大学学报(医学版) 第 42 卷第 6 期第 656 页 Acta Med Unív Sci Technol Huazhong Dec. 2013 2013 年 12 月 miR-218 靶向 Wnt2B 抑制卵巢癌细胞转移侵袭功能铃 饶玉梅l ， 纪妹1 ， 支惠蓉l ， 陈彩虹2 郑州大学第一附属医院 l 妇产科 2 生殖中心，郑州 450052 摘要:目的 研究 miR-218 对卵巢癌细胞 A2780 转移侵袭的影响及可能机制。方法 miR-218 mimics 转染 A2780 细胞，实时定量 PCR 检测转染效率;对转染前后细胞进行划痕及 Transwell 实验，并采用 Western blot 分析 Wnt2B 蛋白 表达的改变。采用双萤光素酶报告基因检测系统验证 Wnt2B 是否为 miR-218 靶基因。结果 A2780 细胞转染 miR-218 mlmlcs 后，细胞运动侵袭功能受到明显抑制，Wnt2B 蛋白表达明显下降，其为 miR-218 直接作用的靶基因。结论 mìR 218 可能通过靶向 Wnt2B 抑制卵巢癌细胞 A2780 的转移侵袭功能。 关键词 :miR-218; 卵巢癌; Wnt2B; 转移; 侵袭 中图分类号:R737. 31 DOl, 10.3870/). issn. 1672-0741. 2013.06.007 miR-218 Inhibits Migration and Invasion of Ovarian Cancer Cells by Targeting Wnt2B Rao Yumei ,Ji Mei ,Shi Huirong et al Department of Obstetrics and Gynecology , The First Affiliated Hospital of Zhengzhou University ,Zhengzhou 450052 , China Abstract Objective To explore the effect of mìR-218 on migration and invasion of ovarian cancer cells(A2780 cells)and its possible mechanism. Methods A2780 cells were cultured and then transfected with miR-218 mimics. The transfection effi ciency was detected by real-time PCR. The migration and invasion of A2780 cells were measured by wound healing and Tran- swell assays before and after miR-218 mimics transfection ,and the level of Wnt2B was detected by Western blot. The dual-!ucif- erase assay was used to determine whether Wnt2B was the target gene of miR-218. R四川ts The migration and invasion of A2780 cells were significantly suppressed in A2780 cells transfe